Celvin® S – advantages

Advantages of the camera-based system Celvin® S compared to film-based detection

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Fig. 1: Comparison of transfer-characteristic lines of a camera-based (blue line) and a film-based (red line) detection.

The capture of chemiluminescent-marked samples (e. g. Western Blots) with photographic films is very sensitive but hardly quantifiable. Figure 1 shows that the characteristic lines of camera-based (blue) and film-based (red) measurements differ dramatically. As you see, the linearity of the camera-based recording of the chemiluminescence emission is excellent whereas the changing of the optical density in film-based method follows a sigmoidal curve with an unsure linearity area.

Furthermore, it is impossible to determine the optimal exposure time when using films. This results in at least some wasted blots and this method is also not GxP-conform.

An advantage of the camera-based system is that no dark chamber is needed compared to a film based system. In this context the follow-up costs of the film-based method must be considered which are not negligible. Even though a modern developing machine can be bought for approx. 6,000 Euro nowadays, you have to include the costs of the consumables like films and chemicals in your calculation as well as the efforts for maintenance, cleaning and disposal. Camera-based imaging systems might be more expensive in purchase but do not need consumables. You need the CL-substrate in any case. The costs for common camera-based detection systems start from approx. 14,000 Euro.

This is the point where we step in: biostep® developed Celvin® S, which is the smallest and most compact camera-based chemiluminescence system for Western Blots with an unbeatable price from 9,350 Euro.

biostep® was successful in creating a camera-based CL system with our self-developed camera EagleIce® and is therefore highly sensitive and exploits the light emission of the CL-reaction in an optimal manner.

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Fig. 2: Typical course of the light emission as a result of the chemiluminescence reaction

The detection sensitivity of a CL system SCL depends on the parameters efficiency, light quantity and device constant and can be concluded in the following formula:

SCL = η * ν * κ

whereas

SCL Detection sensitivity of a chemiluminescence system
η Efficiency of a chemiluminescence reaction in the substrate (quantity of emitted photons per reaction and time unit)
ν Emitted light quantity during the course of reaction
κ Device constant of the detection system

 

As you can see, the efficiency η of the reaction (CL substrate Lumixx®) plays a role as well as the device with the constant κ (EagleIce®).

In the following topics we inform you about chemiluminescence, the technique of Celvin®, its size, the especially developed CL-substrate Lumixx®, the feature white light and the software SnapAndGo, created by ourselves.